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Amputee’s rehabilitation is a challenging procedure which requires expertise especially in the selection of prosthetic components based on amputee’s need and satisfaction. The method of attachment of prosthesis to the residual limb (suspension) and socket fitting is a critical issue in the process of providing an amputee with prosthesis. Different suspension methods try to minimize the pistoning movement inside the socket to enhance the amputee’s gait and satisfaction. The ICEROSS Seal-In® X5 and Dermo® Liner by Ossur are new suspension liners that intend to reduce pistoning between the socket and liner. Since the effects of these new liners on suspension during ambulation are unclear, this study aimed to evaluate the pistoning effect and satisfaction of these liners on transtibial amputees.