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Antibacterial activity of various fruits orange [C.reticulata] pomegranate [Punica granatum], grapefruit (Citrus paradisi) and lemon (Citrus limon ) were evaluated against pathogenic bacterial and fungal strains namely Salmonella typhi, Streptococcus epidermidis, Staphylococcus aureus, Bordetella bronchiseptica and Candida albicans. The agar well method was used to evaluate the MIC (minimal/minimum inhibitory concentration), MBC values (minimum bactericidal concentration) and growth inhibition zone diameters of bacterial and fungal growth at different concentrations of the nutrient agar medium and potato dextrose agar, results were compared with Amoxicillin and Amikacin, a commercial antibacterial agent. Fruit juice of C.limon showed highest antibacterial activity followed by fruit juice of P.granatum variety. Fruite juice of C.reticulata and C.paradisi exhibited least antibacterial activity. Our study concluded that among, all fruit juices citrus fruit C. limon were found to be highly bactericidal, as it has shown lowest MIC and MBC values and high growth inhibition zone diameter in comparison to antibiotics against .This study reveals the higher antimicrobial activity of C.limon