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This report describes the certification of the equivalent spherical diameters of silica nanoparticles suspended in aqueous solution, Certified Reference Material (CRM) ERM-FD100®. The CRM has been certified by the EuropeanCommission, Joint Research Centre, Institute for Reference Materials and Measurements (IRMM), Geel, BE. The intended use of this ERM-FD100 is to check the performance of instruments and methods that determine theparticle diameter of nanoparticles (particle size ranging from approximately 1 nm to approximately 100 nm) suspended in a liquid medium. It is available in 10 mL pre-scored amber glass ampoules containing approximately 9 mL of suspension. The CRM was prepared from commercially available colloidal silica (Koestrosol 1530, Chemiewerk Bad Koestritz GmbH, DE).